Linkage analysis and you can QTL mapping from inside the an excellent tetraploid russet mapping populace away from potato

Linkage analysis and you can QTL mapping from inside the an excellent tetraploid russet mapping populace away from potato

Genome-wide single nucleotide polymorphism (SNP) indicators coupled with allele dosage information enjoys came up given that a robust product for studying advanced traits during the grown autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48). Up until now, this approach might have been effectively put on the new identity away from quantitative attribute loci (QTLs) hidden very heritable qualities such disease resistance, however, largely unexplored to own qualities that have advanced habits off genetics.


In this study, an Fstep one tetraploid russet mapping population (162 individuals) was evaluated for multiple quantitative traits over two years and two locations to identify QTLs associated with tuber sugar concentration, processing quality, vine maturity, and other high-value agronomic traits. We report the linkage maps for the 12 potato chromosomes and the QTL location with corresponding genetic models and candidate SNPs explaining the highest phenotypic variation for tuber quality and maturity related traits. Significant QTLs for tuber glucose concentration and tuber fry color were detected on chromosomes 4, 5, 6, 10, and 11. Collectively, these QTLs explained between 24 and 46% of the total phenotypic variation for tuber glucose and fry color, respectively. The QTL on chromosome 10 was associated with apoplastic invertases, with ‘Premier Russet' contributing the favorable allele for fry processing quality. On chromosome 5, minor-effect QTLs for tuber glucose concentration and fry color co-localized with various major-effect QTLs, including vine maturity, growth habit, tuber shape, early blight (Altenaria tenuis), and Verticillium wilt (Verticillium spp.).


Linkage data and you can QTL mapping in a beneficial russet mapping people (A05141) using SNP amount guidance effortlessly understood advantageous alleles and you can candidate SNPs to have effectiveness the fresh accumulation off tuber cutting sugar. Such unique markers has a premier possibility the improvement regarding tuber running top quality. Moreover, new finding of different hereditary designs to own traits with overlapping QTLs during the maturity locus certainly suggests a separate hereditary control.


Having an elaborate polyploid genetics the brand new cultivated autotetraploid potato (Solanum tuberosum L., 2n = 4? = 48) presents novel pressures for linkage mapping and decimal feature loci (QTL) investigation. The recent development of potato solitary nucleotide polymorphism (SNP) arrays provides a collection of genome-greater markers used for the mapping and decimal attribute locus (QTL) data [step 1,dos,step 3,4]bined towards the way to obtain statistical devices that allow the employment out-of allele amount advice (i.elizabeth., how many copies of each allele within certain polymorphic locus), it is now you'll so you can notably improve power regarding SNP pointers to help you find recombination situations and incorporate this particular article towards QTL mapping [5,six,7]. Up to now, this approach has been successfully used to find unique QTLs and you may candidate SNPs for the later blight opposition [cuatro, 8], inner temperature necrosis , Potato virus Y , and agronomic attributes , but largely unexplored to have training running high quality or any other higher-well worth faculties having state-of-the-art heredity.

There's a significance of unit markers associated with tuber control high quality attributes to greatly help your selection of germplasm to own cultivar advancement. Handling high quality is actually determined by tuber glucose posts. The latest accumulation regarding fructose and sugar (reducing glucose) from inside the potato tubers throughout the cold-storage adversely has an effect on the conclusion-explore high quality properties. Reducing glucose content is a complicated trait controlled by multiple genetic and you can environment activities [twelve, 13]. The brand new advanced hereditary architecture on the feature are consistent with the hobby of several enzymes active in the metabolic paths connecting starch description and synthesis so you can sugar creation and you can usage [fourteen, 15]. Hereditary mapping research shows that fry operating top quality and you can reducing glucose is in the hundreds of short impression QTLs [16,17,18,19,20]. Soltis-Kalina ainsi que al. understood a couple significant QTLs getting processor color for the chromosomes step one and you may six for the a diploid mapping population. Now, Braun et al. reported one or two QTLs getting resistance to cool-induced sweetening on chromosomes 4 and you will 6 off diploid potato. Many of these research has already been did to the diploid potato germplasm. not, then research is wanted to best understand the genetic structures away from these types of attributes in the tetraploid height inside the communities produced from russet-skinned potato species.